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Cytometry Experts,
We are doing flowcytometric analysis of nuclear DNA content and ploidity
determination of paraffin embedded archival pathological lymphoma cases in
dogs.We dewax the 30um sections by two changes of xyleneand dehydrate
with sequential ethanol concentrations and distilled water.Finally we
digest the tissue by pepsin and stain by standard PI staining method.
We get nice diploid and aneuploid peaks,however the fluorescence of the
deparaffinized samples is lower than in the fresh material.
Questions:Is this the expected pattern of paraffin embedded tissue?
If yes:what is the explaination?
If not:how can we increase PI uptake in archival lymphoid tissues?
Amnon Farkas
Univeristy of Illinois
School of Veterinary Medicine
Gary Durack
University of Illinois Biotechnology Center
Flow Cytometry Facility
Voice (217) 244-0559
FAX (217) 244-0466
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web