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We're thinking of upgrading our XL machine to 4colour mostly to run
HIV work through. The antibodies we will be using are
Coulter's three colour reagent (CD3-PE/TxRed, CD4-FITC, and CD8-PE)
We hope to combine this with a CD45-PE/Cy5. (maybe DAKO or similar)
Does anybody have any experience of this combination of reagents or 4
colour work in general on the XL?
Any help would be appreciated.
Best wishes
John
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web