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In response to the question of Ulrich Sack in Leipzig, elastase is a
lysosomal endoproteinase, which also occurs as a secreted form. It
recognizes and cuts (Ala-Ala)n.
Fluorogenic substrates for elastase are available from Molecular Probes,
Inc.; Eugene; OR 97402; U.S.A.; Tel: (541) 465-8300 or from Molecular Probes
Europe BV; Tel: +31-71-233-431; FAX: +31-71-233-419.
There are two Rhodamine-110 versions available (catalog numbers R-6504 and
R-6506) and there is a chloromethyl version of R-6504, which is catalog
number 6569.
Good luck !
Martin Poot
martin@probes.mhs.compuserve.com
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web