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>I am forwarding a request from a coworker regarding getting a GOOD positive
>control to work in our TUNEL/apoptosis assay. Which nuclease have you
>used (company, ph#, etc) with the best results.
>Thanks again!
>Dan Smith
>San Diego
>e-mail: djs@allp.com (or through the users group)
> ----------
>We have been unable to get the positive control to work in her TUNEL
>protocols. The control involves treating the permeabilized cells with a
>nuclease to nick the DNA and render it TUNEL positive. It must be a problem
>with the enzyme itself since we are registering what we interpret as TUNEL
>positive cells in the treated samples. Could you submit a question to the
>internet group concerning what nuclease and vendor might help us solve this
>problem. We could just start ordering different ones from different places
>but this may give us a more reasonable approach. Long live the NET!
-- ============================================================================== Thomas Delohery | Internet: t-delohery@ski.mskcc.org Manager, Flow Cytometry Core Facility | Phone: (212) 639-8729 Memorial Sloan-Kettering Cancer Center | Fax: (212) 794-4019 1275 York Ave. Box 98 | New York, NY 10021 | ==============================================================================
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web