TUNNEL POS. CONTROL
Angel_Delgado@ftdetrck-ccmail.army.mil
Fri, 12 Jan 96 13:38:35 EST
I have been using the In Situ Cell Death Apoptosis kit from Boehringer
Mannheim for a while now. It seems to work really well. For a
positive control I use 10 units of DNase 1 Amplification grade from
GibcoBRL (part# Y02050). I add the DNase after the cells have been
treated, fixed, and permeabilized. I have been getting approximate
85% positive population. I am using human granulocytes in this study,
but I do not believe DNase 1 is bias to certain DNA. Good luck!!!
NOTE: Apoptosis should always be verified by morphology according to
its cell type.
Angel Delgado
Immunologist
US Army Institute of Surgical Research
Fort Sam Houston
San Antonio, Texas 78234
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
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If you have any comments please direct them to
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PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
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