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In message <199601050422.XAA04144@flowcyt.cyto.purdue.edu> writes:
> Regarding the use of the subdiploid PI method to assess apoptosis
> by flow cytometry:
>
> There are two cell preparatory methods: one analyzes whole cells and
> the other lyses the cells and analyzes the released nuclei.
>
> Gating is critical to determine an accurate percentage apoptotic.
>
> Each tube contains "live" cells, apoptotic cells, debris, and
> clumps.
>
> I use a whole cell method with a linear fluorescence scale and
> use both LS gates [mainly to exclude debris] and FL gates [mainly
> to exclude doublets and clumps].
>
_____________________________________________________________
/ Michael J. Herron University of MN, Dept. of Dermatology /
/ herro001@maroon.tc.umn.edu /
_____________________________________________________________
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web