apoptosis by flow
hwortis_sup@OPAL.TUFTS.EDU
Wed, 20 Dec 1995 23:39:58 -0500 (EST)
Is this a problem that other people have solved?
We have been using flow to analyze apoptosis in lymphocytes. We
have been using P.I. staining and using either ethanol or hypotonic lysis
to obtain nuclei and stained nuclear fragments. The problem is whether
to use FSC/SSC gates. If the answer is yes, which particles do you omit
from the gate?
It seems that there is a real problem of analysis. Each
apoptotic nucleus may form several fragments. Therefore there
is no defined relationship between the number of fragments and the number of
apoptotic cells. Do other flowers recognize this as a problem? If so
how do you solve it?
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
