Re: Disappearing monocytes
Howard Shapiro (hms@shapirolab.com)
Thu, 28 Dec 1995 08:42:01 -0500
>The (prepped whole blood) samples run and
>accumulate normally, until he increases the sample rate. Once the rate is
>above 1000-2000 per second, the monos "disappear" ! Reducing the rate
>again brings the monos back into the accumulation. We think this might be
>due to the monos binding to the sample tubing, and/or other surfaces.
>
Monos tend to settle faster with time; twenty years ago, the Block group
noted variations in differential count during different intervals when
running a single sample. However, the appearance and disappearance you are
reporting may be related to the electronics (note the ongoing thread about
mystery side scatter signals in FACScans), even though the ESP should be
capable of dealing with the data rate. I would at least look into that.
--Howard
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
