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We consitently obtain two peaks in the DNA histogram. A
lower peak from the lymphocytes and a peak from the
granulocytes with about 30% more fluorescence.
If we use a detergent method to prepare unfixed nuclei,
there is only one peak in the DNA histogram.
The only reference I can find to a differential staining of
lymphocytes and granulocytes is by Stokke and Steen
(Cytometry 8, 576-583, 1987) who observed a difference
when they stained formaldehyde fixed PBLs with 7-AAD.
Has anyone else observed this effect? Does anyone know how
to persuade the the two types of cell to take up the same
amount of PI?
The reason for this esperiment is that I want to fix bloody
tumour samples for a Tdt assay and I do not want to be
confused by two peaks from the normal cells.
Michael Ormerod
preferred email address: 100537.2462@compuserve.com
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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