Stephen P. Perfetto
Walter Reed Army Institute of Research
1600 East Gude Drive
Rockville, MD. 20850
_______________________________________________________________________________
Subject: Krypton Laser for GFP?
From: Pizzo@nso1.uchc.edu (Pizzo;Eugene) at Internet_Gateway
Date: 11/13/96 7:33 AM
All Colleagues,
A researcher here at UCONN is considering using the 413nm violet line
of a Krypton laser to excite mutant GFP. This researcher has been
somewhat dissatisfied with our results using 488nm excitation on the
FACStar Plus (by the way we've been using a 500 EFLP in Fl1 to
capture the emission). Can anyone enlighten me as to the worthiness
of this approach? Is there a new GFP out there or is 413nm excitation
really significantly better?
Gene Pizzo/UCONN Health
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