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A researcher here at UCONN is considering using the 413nm violet line
of a Krypton laser to excite mutant GFP. This researcher has been
somewhat dissatisfied with our results using 488nm excitation on the
FACStar Plus (by the way we've been using a 500 EFLP in Fl1 to
capture the emission). Can anyone enlighten me as to the worthiness
of this approach? Is there a new GFP out there or is 413nm excitation
really significantly better?
Gene Pizzo/UCONN Health
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web