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An investigator has asked me to look into DNA labelling index analysis for
multiple myeloma patients. There is literature which suggests flourescent
labelling of plamsa cells, along with BrDU, and counting the S phase plasma
cells, is very prognostically significant.
The problem is that the investigators have been told that this can only be
done by flourescent microscopy - "it doesnt work on flow" - Naturally I
reject any such statement as ill-informed :-)
I hope I'm right ... can anyone help me with a flow based protocol ??
Thanks in advance
Peter Chapple
Senior Scientist
Peter MacCallum Cancer Institute
Melbourne AUSTARLIA
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web