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We had a similar problem with the FACStar Plus and found that the
deflection plates were upside down!
Yes, the angle does make some difference, but the longer part of the
plates must be UP towards the nozzle.
We run with the left & right controls at Max and the streams are about
centre of each collection tube. (cannot make it go near the outer edge
of collection tubes)
Good Luck, Joseph.
Joseph Webster (O.I.C. Flow Cytometry)
Centenary Institute of cancer Medicine & Cell Biology
Locked Bag No.6, Newtown, NSW 2042, AUSTRALIA.
Ph: 61-2-9565-6110 Fax: 61-2-9565-6101
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web