 |
 |
 |
 |
 |
For example, 70 um, 27,500 Hz, 12 drops, 3 volt amplitude, the distance
will be around 3.8 mm.
http://facs.scripps.edu/optplus.html
See also:
Flow Cytometry: Intrumentation and Data Analysis,Eds. Van Dilla, Dean, Laerum, &
Melamed, 1985, Academic Press.
Based on the above, I see about TWICE the deflection distance with the optimal
droplet formation than what I see with BDIS settings.
This does slow down the Jet Stream, so sort speed may be slightly effected.
Dennis
*****************************************************************************
* Dennis J. Young Voice : (619) 822-0407 *
* Flow Cytometry Core Facility FAX : (619) 822-0412 *
* University of California, San Diego USA e-mail: djyoung@ucsd.edu *
*****************************************************************************
_____________________________ Reply Separator _________________________________
Subject: Distance between sort streams
Author: yagrawal@messi.uku.fi at @UCSD
Date: 9/12/96 8:25 AM
Hi everybody,
I request some advice regarding sorting.
Question: We use a FACStar for sorting. However, inspite of my best
efforts I am not able to get the Left and Right Streams to separate much
from the center stream. As a result the sorted cells miss the collection
tubes. (The left and rt. stream deflection controls are turned to max).
- Does the angle between the 2 charged metal plates (which is adjustable)
have anything to do with it ?.
- Any other suggestions would be welcome.
Many thanks in advance.
Yash Pal Agrawal
Dept. of Clinical Chemistry,
Kuopio Univ Hospital,
Finland.
>-- Saved internet headers (useful for debugging)
>Received: from flowcyt.cyto.purdue.edu by mail.ucsd.edu; id LAA08417 sendmail 8
>Received: by flowcyt.cyto.purdue.edu (940816.SGI.8.6.9/930416.SGI.AUTO) for cyt
>Received: from messi.uku.fi by flowcyt.cyto.purdue.edu via SMTP (940816.SGI.8.6
>Received: from localhost by messi.uku.fi (AIX 3.2/UCB 5.64/4.03) id AA
>Date: Thu, 12 Sep 1996 08:25:20 +0300 (WET)
>From: Yashpal Agrawal <yagrawal@messi.uku.fi>
>To: Cytometry M
>Subject: Distance between sort streams
>Message-Id: <Pine.A32.3.95.960912081620.136984A-100000@messi.uku.fi>
>Mime-Version: 1.0
>Content-Type: T
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web