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Ch = (ch_dec)Log(S/k)
where
Ch = observed channel
ch_dec = # of channels in one decade
S is origional signal
k is a scaling factor (arbitrary)
Solve for S:
S = k * 10 **(Ch/ch-dec)
For four decades and 0-1023 channels,
ch-dec = 256
If k = 1, signal range is 1 - 10000,
For a different signal range, choose a different k.
Of course, this all depends on the log amp being four
decades and having a true log character.
-Marty Bigos
Stanford Shared FACS Facility
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web