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>>> "BORTH NICOLE" <BORTHN@edv2.boku.ac.at> 9/5/96, 09:45am >>>
Dear Steve,
you asked for information about PerCP. We tried using it, but don't
any more, because PerCP bleaches rapidly at laser powers higher than
50mW. As we have a 5W Argon laser the lowest power we can get out of
it is 60mW. The die bleaches so quickly then, that you see hardly any
specific fluorescence. If you have a weaker laser that should be
fine, however. BD knows about this, but (at least at the time)
did not mention it in any of their product information.
Kind regards
Nicole BorthNicole Borth
University of Agriculture
Institute for Applied Microbiology
Muthgasse 18
1190 Vienna, Austria
Tel. +43/1/36006/6232
Fax. +43/1/369 7615
email: borth@mail.boku.ac.at
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web