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One note of caution: One researcher was staining CD3 PerCP and looking at
FITC intracellular staining, using alcohol to permeabilize. The PerCP
staining disappeared.
We have also used the tandem conjugates, PE+Cy5. Usually some slight
compensation is needed with these reagents.
Nan Harvey
University of MS Medical Center
Jackson, MS
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web