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Other site specific modifications were done to enhance the TSO's daily
monitoring and setup.
For the first time in 9 months the system can be turned on, the same DDF
used, same System pressure etc and obtain the same Drop Delay as the past
few days. I can now obtain purities >99% at rates of 10k/sec plus. Sort
streams are visable from 5 feet away (tight streams).
A test sort last Friday using a ficolled Bone Marrow sample of about 15
million cells was stained with CD3-Fitc, CD34-PE, CD45-PCP. CD3+CD45+
and CD34+CD45+ cells were sorted into seperate tubes. The sample was
sorted at a rate >10k/sec. The initial CD34 % was 1.3 and the CD3 was 7.7.
The sorted CD3 were >98% pure. The CD34 sorted cells were >95%. The
recovery was about 52%. These results were better than expected with the
problems I had been experiencing with this system most of this year.
The problem is fixed. I just need to tweak it.
I would like to hear from anyone else with a TSO or who is going to
purchase one.
Thanks to BD , Alfonso discovered the heat problem and John Cardot and
Larry Duckett modified the system to meet my particular needs.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web