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We are interested in attempting to identifying Th1 and Th2 lymph node
lymphocytes of mice using intracellular cytokines by flow cytometry. Could you
provide details about how you isolated/purified lymph node T cells? Do you
have information about the proportions of cells with IFNg vs IL-4 and possibly
cells coproducing these cytokines? Has this work been published and if so
where?
Thank you for your help,
Ralph Smialowicz
U.S. EPA
RTP, NC 27711
USA
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Content-type: MESSAGE/RFC822
From: Mikael Vestberg <Mikael.Vestberg@inflam.lu.se>
Subject: re:re: intracellular IL4 and IFNg
To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
Message-id: <960531.180050.25860@macpost.lu.se>
Autoforwarded: false
Content-type: TEXT/PLAIN
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Delivery-date: Sat, 1 Jun 1996 19:01:17 EST
Importance: normal
We have done analysis in mouse lymph node T cells=20
after in vitro culture. After 4 days or more of=20
culture w ConA and then a 6 hour PMA/ionomycin=20
treatment in the presence of monensin we see=20
abundant IFN gamma and some IL4. They are not=20
expressed in the same cells.
We use 50 ng/ml ConA and 50 ng/ml PMA and 1 ug/ml=20
ionomycin in the presence of 3uM Monensin.
Mikael
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web