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Another thing worth mentioning: Rh123 has been used to measure 2
different things, which seem sometimes to be confused (or at least
confusing) in the literature. When used to stain cells under
conditions that permit dye efflux (i.e. stain, wash, incubate @ 37o),
it can be used to measure activity of the MDR gene product
p-glycoprotein which actively pumps the dye from the cell. When used
at higher concentration, or under conditions where efflux is blocked,
Rh123 has been used to measure mitochondrial activity (since this
charged low molecular weight lipophilic dye preferentially binds to
mitochondria).
Albert D. Donnenberg, PhD
Associate Professor of Medicine
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web