TNF receptors

Maurizio Mezzetti (mezz001@IT.net)
Wed, 7 Aug 1996 10:07:24 +0200

Hello everybody!
Has anybody had experience in detecting mouse membrane TNF receptors by flow
cytometry?
We have antibodies against the Type I and Type II human TNF receptors (TNFR)
and have found no reactivity with a mouse fibroblast cell line (A9 cells)
which is widely used for detecting TNF bioactivity, and therefore expresses
at least the Type I TNFR. It seems that we'll have to buy anti-mouse TNFR
MAbs, any particular suggestion regarding a specific clone, e.g. high
affinity binding? Any reference regarding expression of these receptors on
A9 cells would also help a lot!

Thanks for the help

Daniela Karrer
Istituto Di Ricerca C. Serono
Via di Valle Caia 22
00040 Ardea, Roma ITALY
tel +6-919491
fax +6-91949203
e-mail: mezz001@pn.itnet.it

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu

Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu