RE: Antibody conjugation

H.-G. Kreysch (kreysch@merck.de)
Wed, 07 Aug 1996 14:47:14 +0200

In our lab different murine (and one humanized) monoclonal antibodies were
labelled using FITC "isomer I" (e.g. Molecular Probes F-1906) with good
results (protocol available on request). Aliquots of labelled antibodies are
stored at -70=B0C and - in our experience - stable for many years.
In one case binding properties of an antibody were lost due to the
conjugation procedure.

------------------------------------------------------------------------
H.G. Kreysch
Merck KGaA
Biomed Fo/IMO - A25/410 Tel.: (49)6151 - 72 7323=20
Frankfurter Str. 250 FAX: (49)6151 - 78 1332
D-64271 Darmstadt E-Mail: kreysch@merck.de

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu

Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu