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Samples with moderate to low percentage apoptosis ran very slowly.
Samples with high percentage apoptosis ran much faster. Could the increase
in flow rate be due to fragments of nuclei being detected by the flow
cytometer?
I set the cytometer so that the G0/G1 peak fell at channel 250 (linear
scale) on the FL2-A plot. For analysis, I gated on the singlets from the
FL2-W vs. FL2-A plot.
Should I be thresholding on FSC instead of FL2? If I threshold on FSC,
where should the cut-off be? Any thoughts on the matter will be
appreciated.
Thanks!
Nan Harvey
University of Mississippi Medical Center
Jackson, MS
nharvey@fiona.umsmed.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web