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Something else you may want to consider for evaluation of the
oxidative burst is using the dihydrorhodamine assay. While I wouldn't
recommend using it concurrently with FITC zymosan, it is a fast, simple
assay (about an hour, from start to finish), and is very specific for
superoxide and/or hydrogen peroxide production.
Here is the reference...
Flow Cytometric Analysis of the Granulocyte Respiratory Burst: A
Comparison Study of Fluorescent Probes.
Journal of Immunological Methods 178 (1995): 89-97
Sarah Vowells
svowells@nih.gov
NIAID
National Institutes of Health
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web