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Ray: The problem you describe is actually predictable based upon the
spectral properties of thiazole orange (see Lee et al, Cytometry 7:508,
1986 or editions of Howard Shapiro's excellent text). TO emits further
into the spectral range of PE relative to FITC. If you need to do two
color work with TO, you will need to use a Moab signal in the red range,
such as tri-color or PerCP.
Bruce H. Davis, M.D.
****************************
Director of Analytical Cytometry
Dept. of Clinical Pathology
William Beaumont Hospital
3601 W. 13 Mile Rd.
Royal Oak, Michigan 48073-6769
810-551-5137
FAX 810-551-8057
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web