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{original cells in the mixture by flow, after a period of time lasting
around
{two hours. So, the question is this: which dye would any of you esteemed
flow
{gurus recommend for this, which will:-
{
{ Readily be taken up by viable cells.
{ Not be removed by washing immediately afterwards.
{ Persist for at least two hours, without serious loss of
fluorescence.
{
{ Potential excitation wavelength for the dye to be used is not a
{problem, as the analysis will be performed using a FACStar+ with a choice
of
{488nm., UV & dye laser excitation available.
{
{ Let the torrent of replies, suggestions, comments, abuse, gossip,
{humour, money, beer, etc. flow (pun intended) ...
{
{Best wishes to all,
{
{"Zip".
{>>>--->
{
{UTSWMC, Dallas, TX.
{
{
{
{
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web