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Zip,
We use biotin to label red cells for in vivo tracking which
you may find suitable.
Labelling is simple : 0.3pg biotin / (red) cell. NHS-LC-Biotin,
Pierce,Rockford,IL in 0.9%NaCl for 10 mins at room temperature.
Wash twice before use.
It is long lived on RBC's - we have seen it many weeks later after
reinjection in vivo.
Detection with streptavidin-FITC gives a good signal to noise ratio -
we detect 1 in 1000 easily with 3% precision.
If you have sufficient material and FACStime going down to 1 in 100000
should be possible.
Details in "Red Cells and Reticulocytes" Chapter 8 ,Flow Cytometry :
Clinical Applications. Ed M.Macey (Blackwell).
Regards,
Terry.
Dr. Terry Hoy
Principal Research Officer
Department of Haematology Telephone 44 1222 743458
University of Wales College of Medicine FAX 44 1222 744655
Heath Park E-mail hoy@cardiff.ac.uk
CARDIFF CF4 4XN http://www.cf.ac.uk/uwcm/hg/hoy/index.html
U.K.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web