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Dr. Sharp,
Though I believe it is possible to obtain good chromosome
resolution when exciting with the 458 nm laser first, I would not
recommended it.
I prefer triggering on UV excited fluorescence since it is brighter
(usually 2-4X) and exhibits better chromosome peak resolution than
fluorescence that results from excitation at 458 nm.
=====================================================================
John J. Fawcett, fawcett@lanl.gov
Life Sciences Division, LS-3 505/667-9633 : office(vmail)
Los Alamos National Laboratory 505/667-2750 : LS Business Unit
P.O. Box 1663, ms M888 505/665-3024 : FAX
Los Alamos, NM 87545
USA
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web