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I have a user that wants to couple protein-ligands to fluorescent latex
particles (Molecular Probes Fluospheres 0.01 um) and use them in binding
studies to the receptor on the cell-surface.
Is there anybody out there who has experience with this ?
Questions are in particular:
1. How to couple the protein to the fluospheres (Molecular Probes didn't
include enough information on their datasheets - Martin let me know if you
have more)...
2. How to block the free binding sites (BSA in excess ?)
3. What's the best size for the particles (is 0.01 um to small ?)
4. How to wash the cells or not before the acquisition ?
etc...
Anyway, any hints would be appreciated...
Thank you very much in advance,
Matthias
______________________________________________________________
Matthias Haury _/_/_/ _/_/_/ _/_/_/
Flowcytometry - Immunology _/ _/ _/ _/ _/
Institut Pasteur Paris _/ _/_/_/ _/_/_/
Email: mhaury@pasteur.fr _/ _/ _/
Tel: 33 (1) 40 61 31 29 _/_/_/ _/ _/
Fax: 33 (1) 45 68 86 39 INSTITUT PASTEUR PARIS
______________________________________________________________
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web