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Indeed, BDIS recommends using nozzles with a diameter that is four times
larger than the diameter of the largest particle sorted! A 200 micron tip
is available for the MacroSORT option (largest nozzle is 400 microns). For
a 70 micron tip, I use a 20 micron mesh; admittedly not quite 4:1, but at
least it gets out the visible clumps, pieces of tissue, etc. :)
/\/\/\_ Eric Van Buren, vanburen%flovax.dnet@rocdec.roc.wayne.edu
\ \ \ Karmanos Cancer Institute and Wayne State University,
\_^_/ Immunology & Microbiology. Detroit, Michigan.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web