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>
> I would like to stain for TdT in Acute Leukaemias using flow
> cytometry. Any references/methods/reagent information would be most
> helpful.
>
> Thanks
> Joy Mundy
> Dept. Human Immunolgy
> Institute of Medical & Veterinary Science
> Adelaide, South Australia 5000
> Ph 61-8-2287476; Fax 61-8-2324092
> jmundy@immuno.imvs.sa.gov.au
>
Hi, I have worked a lot on flow TdT in acute leukemias, particularly
AML. We have summarized a good deal of our efforts in a recent paper uin
Leukemia & Lymphoma, I will send it to you: Meenan et al, Leukemia &
Lymphoma Vol 22:265, 1996. We are getting the best results using the
Caltag Labs fixation/permeabilization kit - in combination with the
FITC-conjugated monoclonal anti-TdT antibody from Supertechs, Inc., Cat#
6600, this method works very well also in AML. TdT detection in ALL is
never a problem, it's TdT in AML that usually is difficult because of the
lower fluorescence intensity. Let me know if you need additional
information - the paper should help you though.
Elisabeth
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web