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It seems CNFDA was available from molecular probes a few years ago
but it is listed as "to be discontinued when our current stocks are
depleted" in ed 5 (1992-4) of the handbook - and not listed at all in
the 1995/6 UK price list.
Others mentioned TO-PRO-3, (and TO-TO-3). I've tried TO-PRO-3 with
yeast and the results are certainly believable. I am a bit concerned
about stories that it may enter viable cells too. I analysed my
samples immediately after adding the dye and didn't detect a problem
with staining of viable cells - although in the flow world it isn't
always easy to analyse immediately ;-)
Another suggestion was carboxy SNARF-1 AM acetate ("a red excitable
pH indicator that is only cleaved to fluorescent SNARF by live
cells"). I haven't followed this lead any further yet.
DiTBAC4(5) was mentioned here on the list.
More than half of the responses were asking for this information so I
would assume that there is a lot of interest in red excitable dyes
(hint to any dye manufacturers out there!!!).
I'll also point any interested parties to the URL
http://pcfcfh.dbs.aber.ac.uk/aberinst/mcytmain.htm#7
where I have begun to compile a list of 633-excitable dyes.
Many thanks again,
Haze
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| Dr Hazel Marie Davey hlr@aber.ac.uk |
|Sefydliad y Gwyddorau Biolegol*Inst. Biological Sciences|
|Prifysgol Cymru * University of Wales|
| ABERYSTWYTH, Ceredigion, CYMRU / WALES SY23 3DA |
| http://pcfcfh.dbs.aber.ac.uk/index.htm |
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web