Synchronicity
Jose Lucas Alves Neto (CGroves@infonet.tufts.edu)
Mon, 8 Jul 96 15:28:34 EDT
Greetings,
A researcher here has a slow growing hepetocellular carcinoma cell line, he
wishes to synchronize in G0/G1. I haven't noticed much of a change in the
cell cycle (staining with PI), even though he treated with mimosine at a
concentration of 300uM for 20 hours. The "baseline" (untreated) cycle is
approx. 30 percent in G2/M. I suggested varying concentrations and incubation
times, but a concern is the toxicity of the treatment to the cells. The
protocol he has been trying to follow is FEBS Letters vol. 318, number 2,
1993. I would appreciate if anyone had any other ideas or literature sources.
Cheers,
Chris Groves
Tufts University School of Medicine
Boston, MA
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
