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Both AMCA and Cascade Blue are UV excitable and can be conjugated to antibodies
(or avidin). Unfortunately, they are quite dim--and will work really only for
the brightest antibodies (i.e., high antigen density). CasBlu-Avidin should
also work reasonably well. CasBlu reactive dye is sold by Mol Probes, and the
conjugation is as easy as FITC.
If you have the option, you can get one of the new Krypton lasers which have 406
nm emission lines--these efficiently excite Cascade Blue, and make CasBlu
conjugates as "bright" (over autofluorescence) as FITC conjugates.
mr
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web