UV excitable ab conjugates

Steven Weintraub (weintrau@visar.wustl.edu)
Tue, 2 Jul 1996 15:20:16 -0500

We are trying to use the enhanced green fluorescent protein (GFP) to
identify transiently transfected cells for cell cycle studies. We want to
quantitate DNA in the cells with propidium iodide and assess for new DNA
sysnthesis with BrDU. Several procedures that we have used to permeabilize
the cells to allow for PI or BrDU antibody uptake inactivate the GFP. Has
anyone worked out a method for using GFP in this manner?

Steven Weintraub, M.D.
Departments of Internal Medicine and
Cell Biology and Physiology,
Washington University School of Medicine
at Washington University Medical Center
Campus Box 8052, 660 S. Euclid Ave.
St. Louis, MO 63110-1093
(314) 362-8964 FAX: (314) 362-8987

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu

Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu