re: Excitation of APS by UV (?)

Beisker Wolfgang (beisker@gsf.de)
Thu, 4 Jul 96 17:51:48 MEZ

Some years ago we had a similar problem, but on very weak fluorescence. We
finally found out, that the laser plasma of the UV laser was exciting some
fluorescence, because it has enough intensity in the blue and red. May be,
that is responsible for it. To check, you can use a UV filter such as UG11 to
block most of the plasma light.

But that is just an idea, better check the fluorescence of the beads in a
fluorescence spectrometer, may be the material of the beads itself
fluoresces! With UV you can nearly excite everything.

Good luck

Wolfgang

Flow is Fun !!!!!!!!!

Dr. Wolfgang Beisker phone: (49) 89 3187 2842
GSF - AG Durchflusszytometrie FAX: (49) 89 3187 3349
Ingolstaedter Landstr. 1 EMAIL: Beisker@gsf.de
D-85764 Oberschleissheim
Germany

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu