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I wouldn't no about the excitation level of APC at UV wavelengths,
tails can reach far. Another check you should do is running
non fluorescence beads of same size as the APC beads. Just a double
check for scatter light penetration through your filters.
Robbert van Leeuwen
Flowcytometry / Applied Optics Group
University of Twente
The Netherlands
Robbert@top.tn.utwente.nl
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web