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> I have an investigator who has been obtaining peritoneal macrophages from
> mice by lavage, culturing them overnight with TNF, and then looking by
> flow for Ia expression. We weren't getting much expression so I had him
> stain with PI at the same time. It turns out that the vast majority of
> the cells are dead. He has tried culturing in suspension or letting the
> cells adhere and using cold to detach them. Does anyone have a method of
> detaching that is easier on the cells and still leaves surface markers
> intact? Any advice is greatly appreciated.
>
> Carol
>
> -------------------------------------------------------------------------
> ---- Carol Oxford _| _| _|_|_|_|
*******************************************************************
*Peter Openshaw (p.openshaw@ic.ac.uk) Direct:0171 594 3854 *
*Respiratory Medicine Switch:+44 171 723 1252 x 3854/3 *
*St. Mary's Hosp. Med. School Fax:+44 171 724 7349 *
*Imperial College, Norfolk Place, London W2 1PG, UK *
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web