Re: CD4 (daily variations)

Bruce S. Edwards (BRUCE@lucy.tli.org)
Mon, 1 Jul 1996 09:40:34 MDT

Elaine, a quality assessment study we published a number of years ago
(Cytometry 10:433-41,1989) addressed your question. We evaluated
freshly prepared peripheral blood lymphocytes from 15 healthy volunteers
over a 19 month period with respect to expression CD4 and several
other membrane proteins (CD3, CD8 and CD20). The median coefficient
of variation for CD4 % positive within the individual volunteers was
4.5% (range 3.2% to 7.8%). Hope this is helpful.
- Bruce

*****************************************************************
>
> Can anyone tell me how CD4 values vary from day to day? (ie. one human,
> tested everyday for a week, for a month, etc.) Is there a reference somewhere?
>
> ************************************
> Elaine Kunze
> Flow Cytometry......Image Analysis
> 8B Althouse Lab
> (814) 863-2762
>
>

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu

Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu