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That aside, it may be possible to block the DNA sites (I see GC-like specificity
on chromosomes) and use TO like pyronin. In answer to the specific questions,
the dye is nearly non-fluorescent when not bound to nucleic acids, excites well
at 488 and emits in FL1 with more FL2 that is manageable but a little more than
you would get for fluorescein (see Cytometry 20:218 for some data on SY-38, a TO
derivative). The very high fluorescence at saturation (DNA and RNA) will swamp
FL2 and probably FL3. Spectra should resemble those of TO-PRO-1 (another TO
derivative) in the Molecular Probes catalog (comments Martin?).
Subject: fluorescent RNA specific dyes
Date: 5/23/96 10:33 AM
Does anyone have or know about recent data on a comparison
of the characteristics of different RNA specific dyes
excitable at 488 or 633/635 nm?
What is the specificity (RNA dependent increase or spectral shift of
fluorescence) of thiazole orange in comparison to auramine O,
pyronine Y or others?
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web