detection of mRNA by flow cytometry
Dr. Howard Petrie (h-petrie@ski.mskcc.org)
Wed, 15 Nov 1995 11:42:10 -0400
Dear fellow cytometrists: I am interested in attempting to detect mRNA on
a single cell basis by in situ staining and flow cytometry. I am aware of
a few papers in the literature (globin message), but they are a little
dates now. I am wondering if anyone could share their experience regarding
similar applications. Are biotinylated riboprobes most likely to be
useful? Which biotinylated nucleotides have been used? How can signal
intensity/ background ratios be maximized? I will repost a summary in the
case of multiple responses. Thanks in advance. HTP
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
