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We have a user that will be obtaining plueral and ascitic fluids from patients
with malignant breast cancer that she would like to isolate the cells from and
then run them on flow for DNA content/ apoptosis. She would like to know what
would be the best way to draw this fluid ie, straight or with some anti-
coagulant, how to store the fluid between the time that it is drawn and prepped,
and any other tricks that others more experienced may be aware of.
Thanks again for all the help
Kris
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web