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> I have read the warnings to only use "fresh" parformaldehyde for fixation, but I
> don't recall ever hearing why.
> Could someone explain the sorts of artifacts that old paraformaldehyde will
> introduce? And what exactly IS old?
Good Question.
We make up a 2%pf solution in PBS and keep that in the dark, 4'C. When
needed, we make up the working (user) solution of a 1% pf in PBS - this
1% we make up fresh each time. HOwever, the 2% stock has been made and
stored for up to whenever we need to make it again. That has often been
as much as 6 months.
David
===========================================================================
+ David L. Haviland, Ph.D. Internet:"haviland@kids.wustl.edu" +
+ Washington Univ. School of Med. A.K.A : The Compiler +
+ Dept. of Peds./Pulm. Box 8116 ICBM-Net : Just hit St. Louis +
+ 400 S. Kingshighway &-6 <- User is Brain Dead... +
+ St. Louis, MO 63110 FAX: 314-454-2476 +
+ (314) 454-6076 +
+ ------------------------------------------------------------------- +
+ Will clone and sequence genes for food. +
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web