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> Hello..
>
> I'm just interested in the current washing - cleanup solution that people
> use for cleaning their machines. Our FACScans systematically get clogged up
> because the hypochlorite solution (supposedly 1 %) forms cristals in the
> stock solution.. the only solution is to prepare fresh every day (which I'm
> too lazy to do...).
>
> Is there anyother detergent/solution that people use to cleanup that
> doesn't have this problem ??
>
> Thanks a lot,
>
> Matthias
Becton Dickinson sent us a cleaning schedule involving Extran 300(from BDH)
Basically, it was:
1. Allow the machine to aspirate about 1ml of 4% Extran 300/Distilled
water.
2. Remove tube and aspirate 1ml of distilled water.
3. Leave tube in position and shut down machine with water still
in tube.
I have found no crystallisation problems with this method.
Eric, Edinburgh UK
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web