TdT positive cell lines
Tom Mchugh (mchugh@pangloss.ucsf.EDU)
Mon, 23 Oct 1995 15:58:29 -0700 (PDT)
We currently use the cell line RPMI 8402 as a TdT positive control both
in flow and on cytopreps (we use RAJI as a negative line). Our cultured
cells are losing their TdT and the new aliquot we purchased from the
Coriell Mutant Cell Repository is TdT negative by flow and on cytoprep
(by monoclonal and polyclonal antibody). Does anyone know which
commercial cell line is good as a positive TdT control? I would rather
not buy many to test just to find out which is best.
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
