Re: Right angle scatter with UV laser line?

Hector Nolla (hector@soest.hawaii.edu)
Wed, 11 Oct 1995 14:52:07 -1000 (HST)

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Ray,
What you describe may be ralated to the type of filter you are using to
pick up side scatter signals. If you are using a 488 di or any other non
UV optics with UV excitation the signal is going to be very weak. You may
try using a UV side scatter dichroic filter. I think we got ours from Omega.
Good luck.
Hector Nolla
Dept. Of Oceanography
Univ. of Hawaii

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu