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Ray
> Do you not get right angle light scatter (or very little of it) when
> using the UV line? I was just setting up for an experiment in which we
> will be using UV on our FACS 440. For alignment, I was using some old
> Polyscience beads
> (about 5 micron dia.) that still fluoresce and I didn't see much side
> scatter.
> Should I? Will it be different with cells, i.e., will I see SS with cells
> even though I don't see it with these beads? If not, is it a property of
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web