Keeping large cells in suspension

gap@MIT.EDU
Wed, 09 Aug 95 17:14:15 +1000

Hi All!

Has anyone found a way to keep large particles in suspension for
any extended period of time? The problem I am having is that I am
trying to sort a fraction of 100um particles and these things settle out
of solution after only 10 minutes or so. This makes the sort long and
tedious. I once heard about a food additive called xanthan gum that
would increase the viscosity of the liquid the particles are in. Does
anyone have this or another product working?

Thanks in advance.

Glenn
MIT Flow Cytometry Core Facility
(617) 253 6454
gap@mit.edu


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