RE: DAPI
kukuruga%kasle1.dnet@rocdec.roc.wayne.edu
Thu, 27 Jul 95 17:23:49 -0400
No...DAPI is not a vital stain. Some while back someone told me it was
and we tried it (compared it with H33342). No DNA histogram. I haven't
looked at my Mol. Probes guide yet, but I think it reads as DAPI being
perhaps a "viability" probe -- like PI -- and only dead cells will label.
Whatever....bottom line...you have to fix your cells. If you do, however,
you will find DAPI out-performs H33342 significantly.
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
