Re: Sorting Hybridoma Cells
RCherv@mail-sh.lsumc.edu
Sat, 01 Jul 95 10:39:11 cst
Dear Neal,
You're right...in our experience, there is little correlation
between cell surface Ig and the level of secretion of Ab by hybridoma
cells, although there may be some hybrodomas that can be selected this
way. The best bet to improve Ab production by a hybridoma is to
reclone with limiting dilution (or Autoclone/ACDU if you've got one)
and to screen clone supernatants by ELISA. Of course, you have to
control somewhat for the rate of cell growth of each clone, but good
high-production clones usually make themselves known fairly quickly by
this method. Good Luck.
Rob Chervenak,
Director, LSUMC Core Facility for Flow Cytometry
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
